Induced protein expression in Leptospira spp. and its application to CRISPR/Cas9 mutant generation
Abstract Expanding the genetic toolkit for Dryer Hinge Block Leptospira spp.is a crucial step toward advancing our understanding of the biology and virulence of these atypical bacteria.Pathogenic Leptospira are responsible for over 1 million human leptospirosis cases annually and significantly impact domestic animals.Bovine leptospirosis causes sub